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Monday, August 24, 2020
Saturday, August 22, 2020
Presley essays
Presley expositions At that point, nobody understood that The universe of music and diversion would until the end of time change. It was early afternoon, on January 8, 1935, when Gladys Smith Presley, a poor Mississippi God-dreading sewing machine administrator and spouse of homestead hand Vernon Elvis Presley, brought forth twin children Elvis Aron and Jesse Garon Presley. Covered in a plain Priceville, Mississippi grave, Jesse Garon kicked the bucket inside six hours of birth. Jesses twin, Elvis Aron, was to turn into the most compelling power in the historical backdrop of famous music and a merging power between societies. As the universes generally charming and effective performer of his age, Elvis sold more than 300,000,000 records, and made Today, Elvis is referred to worldwide as The King of Rock n Roll. The down and out with his gracious and respectful ways, designed his well known squirm and early singing style structure the gospel recovery ministers he grew up with at the familys First Assembly of God Church. We were down and out, man, broke, and we left Tupelo short-term, Elvis stated, a statement which embodies his initial life, following his folks from employment to work. In 1939 the absolute joined Presley compensation was $35 per week, when youthful Elvis was taken a crack at L.C. Humes High School in Memphis, Tennessee. After graduation from Humes Secondary School in 1953, while driving a truck for an electric organization, Elvis passed a sign that would lead him to popularity. The sign read, Memphis Recording Service-Make your own records...Four dollars for two melodies. This is the place Sam Phillips of Sun Records discovered his man. In August 1954, WHBQ radio in Memphis discharged the introduction of a Rock n Roll legend with, Thats All Right, Mama. After a fruitless Grand Ol Opry spell, 1955 brought Good Rockin Tonite and Milk Cow Blues Boogie. The flashy individual oversee ... <!
Thursday, July 16, 2020
What Is a Marriage and Family Therapist
What Is a Marriage and Family Therapist Student Resources Careers Print What Is a Marriage and Family Therapist? By Kendra Cherry facebook twitter Kendra Cherry, MS, is an author, educational consultant, and speaker focused on helping students learn about psychology. Learn about our editorial policy Kendra Cherry Updated on June 15, 2018 Bruce Ayres / The Image Bank / Getty Images More in Student Resources Careers APA Style and Writing Study Guides and Tips Couples and families face unique problems, which is why they often seek out help from marriage and family therapists. These professionals are trained to deal specifically with interpersonal issues that arise in individuals, couples, families, and groups. Are interested in a career in this field? Get a better idea of what marriage and family professionals do, where they work and how much they earn in this overview of this fascinating profession. What They Do Marriage and family therapists assess, diagnose and treat mental illness and psychological distress within the context of the marriage and family systems. The professionals provide counseling services in several different areas including premarital counseling, relationship counseling, child counseling and separation and divorce counseling. Some of the typical tasks that a marriage and family therapist might perform on a regular basis include: Diagnosing and treating mental disordersConducting psychotherapy with individuals, couples, and familiesDeveloping treatment plansHelping clients develop new interpersonal communication skillsCollecting information about clients through self-report inventories, interviews, observations, discussions and formal assessments. In addition to providing client services, many professionals in the field spend time marketing their services, particularly if they work in private practice. Completing paperwork and dealing with insurance companies also takes up a significant portion of a therapists time. When working with a client, therapists need to maintain accurate case progress notes, keep a record of evaluations, and note any recommendations for further treatment. So what makes marriage and family therapists different from other kinds of therapists? While they offer psychotherapy and attend to mental health issues, they focus more on how family dynamics impact psychological health. On a daily basis, they might deal with numerous different client issues such as self-esteem, self-harm, depression, anxiety, grief, anger, and relationship problems. Where They Work Family therapists work in a range of employment areas including outpatient care centers, individual and family services, local and state governments and the offices of other healthcare providers. Other common work settings include private practices, inpatient mental health facilities, schools, universities and health clinics. Since marriage and family therapists often have to adapt to their clients schedules, working evening and weekend hours is not uncommon. How Much They Earn As of May 2014, the median annual salary for marriage and family therapists was $48,040. According to the Occupational Outlook Handbook, the highest paying industries for this profession include: State government - $67,380 (median annual wage)Local government - $55,260Offices of other health professionals - $54,240Outpatient care centers - $49,520Individual and family services - $45,600 Training and Educational Requirements The American Association for Marriage and Family Therapy (AAMFT) suggests that the minimum training to become a marriage and family therapist includes a masters degree plus two years of supervised clinical experience. After completing these educational requirements, therapists must also pass state licensing exams. In addition to the basic training and educational requirements, it is important for professionals to have the characteristics that will allow them to succeed in the job. Interpersonal skills: Compassion and empathy are vital. Marriage and family therapists should be good at listening to their clients.Problem-solving abilities: Marriage and family therapists need to be able to deal with complex problems and come up with effective solutions.Ability to build trust: Therapists need to help their clients feel able to trust and share their innermost thoughts and feelings.Organizational skills: They also need to be able to keep track of case notes and other paperwork and manage billing and insurance claims, which is why organizational abilities are essential.Communication skills: Marriage and family therapists also collaborate with others including family members and healthcare professionals, so strong communication skills are a must. Comfort with different technologies can also be helpful. Marriage and family therapists often utilize a range of technology tools including video conferencing, accounting software, multi-line telephone systems, spreadsheet software, email, and medical software. Job Outlook As of 2012, there were an estimated 37,800 marriage and family therapists employed in the United States. The Occupational Outlook Handbook suggests that the projected job growth for the profession will grow at a rate faster than the average through the year 2020. They project an estimated 29 percent growth between the years 2012 and 2022.
Thursday, May 21, 2020
Poetic Analysis of Psalm 19 - 1074 Words
Pslam 19 is a hymn with a focus on Godââ¬â¢s creation and His revelation to the world through scripture. Through this psalm we are able to see how God has chosen to reveal Himself to the world. He chose to reveal Himself through creation and through His Scriptures. This Psalm also defines characteristics of God through how He describes His Word and Creation. The psalm scientifically provides insight as it describes the heavens and the action of the sun. As insightful as the psalm is, itââ¬â¢s important to recognize the poetry that lies on the pages. ââ¬Å"Which is as a bridegroom coming out of his chamber; It rejoices as a strong man to run his course.â⬠(Psalm 19:5 NASB) The simile used in the verse paints a great picture for the sun and how itâ⬠¦show more contentâ⬠¦You have to first understand that the law has authority to trust that it can restore you. This is a great depiction of progressing in meaning. ââ¬Å"Day to day pours forth speech, and night to night reveals knowledge.â⬠(19:3 NASB) This anthropomorphism gives human form to the non-human form of day and night. The human characteristic of pouring forth speech and revealing knowledge tells about what God has created. God has created something so amazing that it reveals part of who He is. Even though God has humans to carry out his message, and to reveal knowledge through this verse we know that it is not the only way God revealed Himself. The overall structure of the Psalm is that it is broken into two halves. The first half of the psalm is Godââ¬â¢s creation. The second half deals with Godââ¬â¢s Word. Though they are both different they both share the common theme of god revealing Himself to mankind. Inside of these halves the author uses different parallelism. In the first verse we see synonymous parallelism, using the heavens and skies to make the point that they are telling of Godââ¬â¢s Work. In the seventh verse we see synthetic parallelism showing the progression from perfection to restoration of the imperfect. This psalm is a difficult psalm to break down due to differences in structure but also in itââ¬â¢s nature. This psalm is about the creator of theShow MoreRelatedThe Book of Job732 Words à |à 3 Pagesdiseases. The author of the book then tried to show in a poetic dialogue the reaction of job himself and the reaction of his friends to the disaster. And in the last chapters we God communicate with Job. ââ¬Å"Job is the first of the poetic books in the Hebrew Bible.â⬠Greater part of the book is filled with poetic style hence; the book of job is categorized under poetry or wisdom Literature along with the other four books known as wisdom Literature (Psalms, the Song of Solomon, Ecclesiastes, and Proverbs)Read MoreLight Shade: An Exegetical Brief of Psalms 272043 Words à |à 9 PagesLight Shade: An Exegetical Brief of Psalms 27 ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ââ¬Å"Light and Salvationâ⬠: An Exegetical Brief of Psalm 27 A. An outline and Structural Analysis! - Enemies and the Fear of the Lord (v1-3)! - The Psalmistââ¬â¢s One Petition (v4-6)! - Distress, but resolved to wait on Jehovah (v7-12)! - Reassurance (v13-14)! ! B. Introduction ! ! The book of Psalms consists of one hundred ï ¬ fty songs and prayers in the Wisdom literature section ofRead MoreEssay on Psalm 903370 Words à |à 14 Pagesstewardship and enterprise.â⬠3 It is also that entrepreneurââ¬â¢s responsibility to make his or her days count by forwarding the kingdom. Psalm 90: Discussion This tension between manââ¬â¢s place in the continuum of Godââ¬â¢s eternity is the theme that characterizes the Hebrew poetic text of Psalm 90. This text begins the fourth division of Psalm, authored by Moses. Historically, Psalm 90 was written after the wilderness experience of forty years; the Exodus generation had perished. According to the book of NumbersRead MorePsalm 104 Thesis Statement Essay3246 Words à |à 13 PagesThemes for Psalm 104 Yahwehââ¬â¢s relationship to the natural world Yahweh exercises his kingly rule over creation, not through mankinds dominance, but through his direct rule and intervention of his Spirit Godââ¬â¢s mighty acts in nature (ââ¬Å"the earth is satisfied by the fruit of His workâ⬠ââ¬â Psalm 104:13) The Positive Outlook On Life ââ¬â Psalm 104:34 How many are your works, O LORD! In wisdom you made them all; the earth is full of your creatures. Psalm 104:24 Hymns A. Hymns in General Read MoreThe Book of Obadiah 2812 Words à |à 11 Pagesas an object of study probably because of its unimpressive dimensions. Yet, it is an archetypical example of prophetic style and content.â⬠The size of the book does not diminish its importance and worth of scholarship and research. CONTEXTUAL ANALYSIS Historical Context In 586 BCE Nebuchadnezzarââ¬â¢s army crushed Judah and destroyed Jerusalem and Solomonââ¬â¢s temple, ending Judahââ¬â¢s existence as an independent nation. Edom as a closely related nation should have helped Judahââ¬â¢s refugees. Instead ofRead MoreEssay on Book of Job2920 Words à |à 12 Pagessame time. Its literary work is written in a poetry sense with a prose format and considered one of the greatest pieces of literature of all time. The Book of Job is one of first book of five generally called The Books of Poetry, which contain Job, Psalms, Proverbs, Ecclesiastes, and the Song of Solomon. The Book of Job is written in the Old Testament of the Hebrew Bible and the main theme that is seeks out is Why does God allow the righteous to suffer? First of all I will be talking about the originsRead MoreRastafarian79520 Words à |à 319 Pagessame condition on any acquirer Library of Congress Cataloging-in-Publication Data Edmonds, Ennis Barrington. Rastafari : from outcasts to culture bearers / Ennis Barrington Edmonds. p. cm. Includes bibliographical references and index. ISBN 0-19-513376-5 1. Rastafari movement. 2. Jamaicaââ¬âReligious life and customs. I. Title. BL2532.R37 E36 2002 299â⬠².676ââ¬âdc21 2002074897 v To Donnaree, my wife, and Donnisa, my daughter, the two persons around whom my life revolves; and to the ancestors
Wednesday, May 6, 2020
The Effects Of Chronic Conditions On A Diagnosis Of Major...
The article, Quantifying the impact of chronic conditions on a diagnosis of major depressive disorder in adult focuses on the background on the diagnosis and how chronic illness can lead to major depressive disorder. The researcher conduct a study at Mayo clinic with patients that were diagnosed with a chronic illness. Researchers found that patients with specific chronic illness such as diabetes and rheumatoid arthritis/osteoarthritis may have a greater change of being diagnosis with major depressive disorder. Ryu, E., Chamberlain, A. M., Pendegraft, R. S., Petterson, T. M., Bobo, W. V., Pathak, J. (2016). Quantifying the impact of chronic conditions on a diagnosis of major depressive disorder in adults: a cohort study usingâ⬠¦show more contentâ⬠¦Psychiatry Clinical Neurosciences, 68(2), 110-119. doi:10.1111/pcn.12106 The article, A descriptive analysis of quality of life using patient- reported measures in major depressive disorder in a naturalistic outpatient setting, focuses on how major depressive disorder affects a persons quality of life. The researcher conducted a cross- sectional study with 319 patients that were seeking treatment for Major depressive disorder at a outpatient hospital. The researcher concluded that, impairment of quality of life increases in a monotonic fashion with depressive symptoms severity(Ishak, Bresee, Greenberg, Fakhry, Christensen, Rapaport, 2013). IsHak, W., Balayan, K., Bresee, C., Greenberg, J., Fakhry, H., Christensen, S., Rapaport, M. (2013). A descriptive analysis of quality of life using patient-reported measures in major depressive disorder in a naturalistic outpatient setting. Quality Of Life Research, 22(3), 585-596. doi:10.1007/s11136-012-0187-6 Mental Health Interventions: Major Depressive Disorder The article, Cognitive-Emotional Training as an Intervention for Major Depressive Disorder, focuses on cognitive emotional training as an intervention tool to assist patient with Major Depressive Disorder. The researcher utilized cognitive emotional training to enhance cognitive control for emotional information processing and targeting components in patients with major depressive disorder (Xiaozhen, Tianmei, Gang, Huali, Qi, Changqing, Xin, 2016). TheShow MoreRelatedThe Diagnostic Criteria For The Depression Essay1358 Words à |à 6 Pagesareas of functioning. C. The episode is not attributable to the physiological effects of a substance or to another medical condition. May include the feelings of intense sadness, rumination about the loss, insomnia, poor appetite, and weight loss noted in Criterion A, which may resemble a depressive episode. Although such symptoms may be understandable or considered appropriate to the loss, the presence of a major depressive episode in addition to the normal response to a significant loss should alsoRead MoreSymptoms And Treatment Of Depression1180 Words à |à 5 Pagescommon mood disorder and has a lifetime prevalence rate of 6-25% in international studies (Carr, 2012). Reliable diagnosis is vital for the study of mental disorders (Fried, Epskamp, Nesse, Tuerlinckx Borsboom, 2016) and with the rising issue that depression is, individuals with multiple chronic diseases can be tackling depression occurring at the same time thus, it may complicate the treatment of these chronic illnesses. However there i s a question of whether it is due to chronic illnesses asRead MoreThe Woman Who Feels Out Of Control Case Study1388 Words à |à 6 PagesWoman Who Feels Out of Control Major depressive disorder (MDD) is a common mental health disorder seen in primary care. It effects all age groups, although the presenting signs and symptoms will vary at different ages. Many clients have somatic symptoms that mask the depression symptoms and make it difficult for physicians to diagnose MDD. Patients with this disorder have difficulty with work and social functioning. There is an increase in comorbidity with chronic illnesses. These clients areRead MoreProzac Nation Essay1540 Words à |à 7 PagesRunning Head: Prozac Nation and Major Depression Disorder Prozac Nation and Major Depression Disorder Raquel H. Sasyn University of Houston Ms. Chastity Farr Clinical Psychology Section - 25673 April 23, 2010 Introduction ââ¬Å"Prozac Nation,â⬠is a best-selling memoir written by Elizabeth Wurtzel in 1994. Based on Wurtzelââ¬â¢s stormy childhood and adolescence, Prozac Nation explores many topics such as major depression, family relations, and drug abuse. The story narrates Wurtzelââ¬â¢s lifeRead MoreBipolar Disorder1126 Words à |à 5 PagesBipolar Disorder Bipolar Disorder In the world today Bipolar Disorder Abstract This article reviews the current state of the literature on the assessment of bipolar disorder in adults. Research on reliable and valid measures for bipolar disorder has unfortunately lagged behind assessment research for other disorders, such as major depression. We review diagnostic tools, self-report measuresRead MoreDifferent Depressive Disorders1397 Words à |à 6 Pages DEPRESSIVE DISORDERS Introduction: Major depressive disorder is characterized by a myriad of symptoms that interferes with a persons daily activities including ability to work, sleep, study, eat, and enjoy once-pleasurable activities. Major depression is a debilitating disease and prevents a person from being functional socially and professionally. Major depressive disorder is known to have significant potential morbidity and mortality, and contributes to suicide, incidences and adverse outcomesRead MoreSymptoms And Symptoms Of Early Onset Bipolar Disorder1742 Words à |à 7 Pages Early-onset bipolar disorder is a chronic mood disturbance that causes dramatic shifts in oneââ¬â¢s mood that is uncharacteristic of their normal mood and behavior. This hereditary behavioral disorder causes mood swings from extreme lows, depression, to extreme highs, mania. It usually occurs in mid- to late-adolescence but can appear as early as elementary school. The unique symptoms of this age group are angry and aggressive outbursts followed by periods of remorse and guilt, declining academic performanceRead MoreComplex Integrated Psychiatric Disorders1475 Words à |à 6 PagesRunning head: COMPLEX DISORDERS Complex Integrated Psychiatric Disorders Kathleen Owens, MSN, FNP-C University of Cincinnati ââ¬Æ' Complex Integrated Psychiatric Disorders Psychiatric mood disorders of such as Bipolar are often complex to diagnosis. Bipolar I is differentiated from Bipolar II by a history of at least one manic episode in a personââ¬â¢s life, with Bipolar II being diagnosed and characterized by a history of major depression with at least one episode of hypomania (Sadock, Sadock,Read MoreEssay On Adolescent Depressive Disorder1476 Words à |à 6 PagesChild Adolescent depressive disorder treatment Today review evidence-based treatment fashion in Children and adolescents. We will also review new Suicide Prevention guidelines Slide three old medication should be considered off-label I have the approval is mentioned 4 Prevalence of major depressive disorder in Children and adolescents 7.7% of 8.8% of children having severe impairment prevalence in children is 2% ulta female Day short is about to Mom adolescent is 4 - 8% would male to female ratioRead MoreEssay on Depressive Disorders1695 Words à |à 7 PagesThere are many ways to effectively treat depression like medications, psychotherapies, and other methods. Furthermore, there are several forms of depressive disorders: major depressive disorder (major depression), dysthymic disorder (dysthymia), minor depression, psychotic depression, postpartum depression, seasonal affective disorder, and bipolar disorder. All of those types of depression are most likely caused by a combination of genetic, biological, environmental, and psychological factors. The
Writing to inform â⬠Poverty Free Essays
Poverty is the greatest problem facing the world today and through the annals of history. The fact that wealth is distributed so unevenly, many would say unfairly is a problem that has occurred for millennia. Whether it be the social stratification of ancient Egypt or that of todayââ¬â¢s world, poverty has always existed and some say, always will. We will write a custom essay sample on Writing to inform ââ¬â Poverty or any similar topic only for you Order Now Ever since there has been poverty, there have been those that have tried to help the poor. There may have been money given, or food offered, there has always been a struggle to combat poverty. However this has always seemed futile, the black hole of poverty in our world has never been closed, and it has never ended. Though, in these modern times, due to our social structure and awareness, there are fewer people suffering from absolute poverty than any other time in history. Every time we enter the town centre, we are surrounded by hordes of collectors, every penny we give leads to the reduction of poverty. Every time we flick on the TV, there are countless adverts telling us to donate. Every time we look in a magazine or newspaper, there are articles telling us of the worldââ¬â¢s ills. Never has there been such a mass awareness, leading to donations, from people all around the world, to people all around the world. You still may be wondering why I have chosen poverty as the worldââ¬â¢s greatest problem. It is because it is the Russian doll of the worldââ¬â¢s problems. The fact that poverty leads to crime, disease, famine, lack of education and pollution truly makes it the roots of the worldââ¬â¢s problems. Everything stems from poverty. The distillation of water is also a major problem. The fact that distillation is an expensive process, requiring many resources to use, leads to a poor quality of water in those countries that canââ¬â¢t simply afford it. The water may carry diseases such as cholera and diarrhea, which leads to the death of many people. This will most likely be infants and the elderly, as they are more susceptible to illnesses. There may also be a lack of food due to the poor quality of the soil as fertilisers may be too expensive. This in turn leads to crime; mothers and fathers may steal to feed their children. People will do anything to survive, even if it is against their basic morals. The fact that this leads to crime truly proves the ââ¬Å"Russian dollâ⬠theory. Poverty has lead to a lack of food, which in turn has led to crime. The fact that poverty is an endless spiral leads it to its longevity in our world. A lack of education leads to poorly paid jobs. As prices rise due to a population increase, the poor are made even poorer. The fact that the human population increases due to the poor having many children to sustain their agricultural work, leads only to an increase in prices. Corporations know of supply and demand. There is always a greater demand if the population increases, and all they must do is increase their prices. Poverty has led to all these things, and these things lead to poverty. It is as if it is a bacterium. Constantly splitting, growing in number. However the modern era of donation maybe the virus that purges poverty from our world. Maybe one day, we will live in a world where wealth is distributed evenly. Where the larger corporations play ââ¬Å"fair,â⬠and the word ââ¬Å"poorâ⬠never has to be used ever apart from in a history lesson. Maybe we can lead to that sort of world; maybe we can be the virus that destroys poverty. We maybe the generation which made poverty history. How to cite Writing to inform ââ¬â Poverty, Papers
Saturday, April 25, 2020
Lab Report and Analysis of Bos Taurus Tissue Sample
Lab Report and Analysis of Bos Taurus Tissue Paper After centrifuging the sample for 5 seconds, the contents were loaded onto a polysaccharide protein gel set t IV for Electrophoresis and stained Accompanies blue for an hour and results were observed the next class period a week later (Gladdening). To determine Protein Concentration of the homogenate, using a ratio, 50 Pl of original homogenate was diluted in 200 Pl of water and labeled dilute homogenate. 30 Pl measurements of 0. 4, 0. 8, 1 . 2, 1. And 20 MGM/ml were added into their own sample of the 3 ml Bradford Assay, 30 of water was added to a separate sample of Bradford Assay and 30 Pl of the dilute liver homogenate was added to its own sample of Bradford Assay and left for 3 minutes. The absorbency of he standards and the diluted homogenate samples were recorded to create a standard curve. From this equation, the protein concentration of my sample was determined. Class averages were also obtained (Gladdening). Y? 0. Xx + 0. 186 , 0. 572 Protein to DNA ratio was calculated by d ividing the determined average protein concentration of the homogenate by the given DNA concentration for all three Boss Taurus homogenates (Gladdening). Original homogenates were used to digest Glycogen. First, 50 Pl of 8 MGM/ml maliciousnesss was added to 50 Pl of homogenate to create a reaction to digest glycogen. Another 50 VI f homogenate was then combined with 50 Pl of 0. 2 M citrate buffer to act as a control with no enzyme to digest glycogen. The reactions were incubated at 37 co for 2 hours and we later observed absorbency (Gladdening). During incubation, homogenate triglycerides were measured by assuming that triglycerides in all homogenates were equal to total glycerol. Glycerol standard sample was given to the class. One sample of 3 ml Triglyceride reagent was heated at 37 co for 5 minutes, then mixed with 30 Pl of Boss Taurus homogenate and incubated for 10 more minutes at the same temperature. Absorbency of the glycerol standard ND homogenates were measured, and converted to concentration of glycerol (Gladdening). ( AWAY homogenate / AWAY of the standard ) x 2. MGM/ml glycerol The average triglyceride amount for each homogenate was determined. To make a standard curve of glucose, 6 samples of 3 ml of Trendier reagent were heated at ICC. After 5 minutes, 30 Pl of 0. 2 M citrate buffer was added to one sample, 30 Pl of 0. 2, 0. 4, 0. 6, 0. 8, and 1. 0 were added to one of the remaining 5 samples of Trendier reagent and heated for 10 minutes at the same temperature. Absorbency of the standards was measured (Gladde ning). Y= . Xx . 0005, RE = . 871 To measure the free glucose and glycogen, 2 samples of 3 ml of Trendier reagent were warmed at 37 Co. We will write a custom essay sample on Lab Report and Analysis of Bos Taurus Tissue specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Lab Report and Analysis of Bos Taurus Tissue specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Lab Report and Analysis of Bos Taurus Tissue specifically for you FOR ONLY $16.38 $13.9/page Hire Writer After 5 minutes, from the digestion of glycogen process, 30 Pl of the maliciousnesss-treated reaction was added to one sample and 30 Pl of the free glucose control was added to the other sample. The samples were heated for 10 minutes at ICC and absorbency was recorded. Concentration of free glucose was calculated when solving for x using the absorbency of the reaction without maliciousnesss with the above equation. The same was done to find concentration of total glucose using the absorbency of the reaction with milliseconds. Total glycogen was then calculated by subtracting free glucose from total glucose (Gladdening). II) Results: Kodak. 250 150 100 75 50 37 25 20 15 10 Figure 1. Protein electrophoresis of Boss Taurus Heart, Kidney and Liver Homogenates. Lane 1 is the protein ladder in Kodak. The heart homogenate is located in lanes 2-5, kidney homogenate in lanes 6-10, and liver homogenate in lanes 11-15. Table 1. Protein and DNA concentrations, and Protein to DNA ratio of Boss Taurus heart, kidney and liver homogenates. Protein Con. Based on class averages. DNA Con. Ratio Heart n=17 4. 53 MGM/ml 1. 05 peg/ ml 4. 1 MGM protein / peg DNA Kidney n=15 4. 97 MGM/ml 5. 79 peg/ ml 0. 86 MGM protein / peg DNA Liver n=14 2. 5 MGM/ml 7. 64 peg/ ml 0. 39 MGM protein / peg DNA Figure 2. Total triglyceride concentration (MGM/ml) in the Liver, Kidney, and Heart homogenates. Class averages shown. Liver n=12, Kidney n=11, Heart n=16. Figure 3: Total glycogen concentration (MGM/ml) in the Liver, Kidney, and Heart homogenates. Class averages shown. Liver n=18, Kidney n=14, Heart n=14. 228600133985 Figure 4: Total free glucose concentration (MGM/ml) in the Liver, Kidney, and Heart Looking at Protein Concentration (Figure 1), the homogenate tissue with the cost abundant concentration was the heart. It shows the most pronounced bands of proteins, especially the 250 Kodak band, as well as the greatest amount of visible bands of proteins. The difference in concentration between Kidneys and Liver homogenates is more difficult to differentiate. Contrastingly, Table 1, which is based on class averages, shows that the homogenate with the highest protein concentration is the kidney as opposed to the heart. Table 1 also shows Protein/DNA ratio, where the heart is the highest homogenate. We can infer that the large amount of protein per DNA could mean that the cells must be larger o accommodate for the abundance of macromolecule. Triglycerides (Figure 2) of each homogenate are a reflection of the amount of glycerol because the absorbency readings of glycerol were converted to triglyceride concentration during the experiment. Here we see that the kidney homogenate had the highest concentration of triglycerides followed closely by the liver homogenate. In order to obtain values for glycogen, free glucose and total glucose concentrations had to be calculated using the glucose standard curve. Once these numbers were known, subtracting free glucose from total glucose came out to the amount of logger. Figure 4 shows free glucose levels, where the liver had the highest amount and the kidney the lowest. Figure 3 provides glycogen concentration, where again the liver has the highest levels, but the heart homogenate has the lowest. To conclude, each cell type of the Boss Taurus had varying levels of macromolecules according to the experiments performed in these labs. Reference: Gladdening, B. , SST. Angelo, C. J. , Krause, M. K. , Tangelo, J. R. , and Valier, L. G. 2014. [Analysis of Macromolecules in Tissue Homogenates of Boss Taurus Apart and Apart]. Bio 11, Biology, Hoofers University.
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